Read culture conditions for jasmonic acid and biomass production by botryodiplodia theobromae in submerged fermentation, process biochemistry on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Some pathological studies on botryodiplodia theobromae pat. Kinetic analysis of the mechanism of action of betaglucosidase from botryodiplodia theobromae pat. However, sutton 5 has adopted the name lasiodiplodia theobromae griff. Partial purification and characterization of a jasmonic.
Botryodiplodia theobromae causes stem rot disease of citrus. Tujuan penelitian adalah mengetahui patogenisitas dan peran toksin dari tiga isolat b. After 5 days, the mycelium was homogenized with distilled water and used for inoculation of the main culture. Corms that were devoid of petioles and shoot material, and which were scraped and stored at ambient conditions rapidly lost weight, as much as.
Morphological, pathogenic and molecular characterization of. The fungal pathogen lasiodiplodia theobromae has been associated with. Lasiodiplodia theobromae pneumonia in a liver transplant. Red globe plants confirmed the pathogenicity of the isolates and revealed differences in virulence among the isolates tested. Botryodiplodia theobromae and its association with musa species. Induced sporulation of botryodiplodia theobromae pat. Desapping placing the stem end of newly harvested fruit into the soil or turf beneath trees should be avoided since infection by l. Lasiodiplodia theobromae name synonyms botryodiplodia ananassae sacc.
There was an invariable association of lasiodiplodia theobromae with aerial as well as underground parts. Business, international butt rots observations fungi, pathogenic diseases and pests kenaf plant pathogenic fungi. Stotzky with 7 figures botryodiplodia theobromae pat. Botryodiplodia theobromae, the anamorph of botryosphaeria rhodina berk. The fungus was also strongly inhibited by benomyl, with radial growth reduced. Morphological and molecular data reveal cryptic speciation in lasiodiplodia theobromae. Muhammad ali khanzada, abdul mubeen lodhi, and saleem shahzad, pest and disease research lab, department of botany. However, the cankers are favored by high temperatures. Materials and methods field layout the field plot has been monocropped to dioscorea. Botryodiplodia theobromae, a common rot fungus, causes serious economic losses in cultivation of many different. Also isolated from ulcerated human cornea, lesions on nail and subcutaneous tissue. Diplodia stemend rot caused by lasiodiplodia theobromae synonyms. Emerging resistance against different fungicides in lasiodiplodia theobromae as the cause of mango dieback in pakistan ateeq ur rehman1, ummad ud din umar 1, syed atif hasan naqvi1, munaza rana latif, sajid aleem khan2, muhammad tariq malik3 and shoaib freed4 1 department of plant pathology, bahauddin zakariya university, multan, pakistan.
Colonization of cashew plants by lasiodiplodia theobromae ainfo. Pdf mango plantations in different areas of sindh were found to suffer from a decline disease showing symptoms of. It is a common post harvest fungus disease of citrus known as stemend rot. Botryodiplodia theobromae is a cosmopolitan and diverse species. Pathogenic association and management of botryodiplodia. Botryodiplodia theobromae, was most commonly associated with dieback and was consistently isolated from various tissues twigs, bark, vascular tissue and fruits of affected plants. Lasiodiplodia theobromae in citrus fruit diplodia stem. Botryodiplodia theobromae and its association with musa. The cellulolytic enzymes of botryodiplodia theobromae pat. Direct microscopy analysis of the bronchoalveolar lavage specimens showed septate hyphae. Calcium nitrate fertilizer effect on anthracnose disease.
A dematiaceous mold was recovered and identified as l. It was also found that injury to the fruits enhanced the chances for infection. Filtrates from cultures of different ages of botryodiplodia theobromae pat. Botryodiplodia theobromae an overview sciencedirect topics. Kuntze 1898 botryodiplodia ulmicola is an ascomycete fungus that is a plant pathogen. This fungus attacks more than 280 species of plants in different parts of the world domsch et al.
Yoshinori asakawa, yoshiaki noma, in comprehensive natural products ii, 2010. The fungus botryodiplodia theobromae strain d 72 1 isolated from cuban oranges was maintained and pre. The fungus is found throughout the tropics and subtropics. However, with this new development of severe dieback, similar to that described recently on other tree crops such as mango and kumquat in other countries khanzada et al. Report by international journal of agriculture and biology. Mango dieback and gummosis in sindh, pakistan caused by lasiodiplodia theobromae. The pathogenicity test showed that the isolates of lasiodiplodia theobromae, caused stem canker and needle blight on araucaria heterophylla. A causal pathogen of black rot disease on kenaf seeds in malaysia.
Lasiodiplodia theobromae is a plant pathogen with a very wide host range. It is also a confirmation of the pathogenicity of l. Postharvest diplodia stemend rot develops from latent. Febs 18452 febs letters 407 1997 105110 partial purification and characterization of a jasmonic acid conjugate cleaving amidohydrolase from the fungus botryodiplodia theobromae silvia c. Plant pathogen causing many tropical fruit diseases including mango twig blight and mango stem rot. Kaur2 1division of plant pathology, sherekashmir university of agricultural sciences and technology of kashmir, shalimar, srinagar, jammu and kashmir, india. Botryodiplodia stem end rot of mango and its control. It is a windborne, leafpenetrating, vascular pathogen, and may have evolved. Read taxol from botryodiplodia theobromae bt 115an endophytic fungus of taxus baccata, journal of biotechnology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips.
Lasiodiplodia theobromae wikispecies, free species directory. Lasiodiplodia theobromae is a phytopathogenic fungus causing gummosis, a threatening disease for cashew plants in brazil. Learn about botryodiplodia theobromae see lasiodiplodia about us. Frontiers biological control of mango dieback disease. Mar 16, 2020 synonym of lasiodiplodia theobromae pat. Leaf and tuber analysis were carried out to determine effect of bounty fertilizer on mineral levels, phytochemical, phenol. The rot fungus botryodiplodia theobromae strains cross infect cocoa. In the mississippi delta, botryodiplodia theobromae placed beneath the bark of sycamore trees incited cankers on 99% of trees inoculated in july, and on 66% of those inoculated in september. The purification and properties of extracellular betaglucosidase from botryodiplodia theobromae pat. Lasiodiplodia theobromae and the roles of insects in dispersal of the fungi. Lasiodiplodia theobromae as a cause of keratomycoses. Among these isolates, 19 12 sa and 7 nsa showed antagonistic. This study investigated infection and weight loss in market samples of ipomea batatas in relation to their phenol and calcium content which confer resistance to infection in plants. Serangan parah penyakit dapat menyebabkan kematian apabila tidak dikendalikan.
Influence of culture media, temperature and lightdarkness on. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. This study aimed at determining the effect of soil amendment and foliarsprayed calcium nitrate on anthracnose disease and tuber rot caused by botryodiplodia theobromae in d. Calcium nitrate fertilizer effect on anthracnose disease caused by colletotrichum gloeosporioides penz. Pdf the rot fungus botryodiplodia theobromae strains cross infect. Their maturation is slow, and involves increased deposition of electronopaque material in the outer region of the original wall of the conidium and the appearance of an electrontransparent interior secondary wall.
Lasiodiplodia theobromae is a common pathogen that occurs on a large number of hosts in the tropics and subtropics. Inoculation tests were performed and the pathogenicity of the organism on its host was confirmed. This study aimed at establishing genetic diversity of b. Botryodiplodia theobromae, dioscorea rotundata, tissue rot, yam introduction a great magnitude of losses of tuber crops resulting from many causes pathological and nonpathological in storage has been reported throughout the world noon, 1978, ricci et al. The biology of botryodiplodia cankers in oaks is poorly known. The active site of betaglucosidase from botryodiplodia. Evaluation of jasmonic acid production by lasiodiplodia theobromae under submerged fermentation kavitha mary jackson, marimuthu ponnusamy and sivakumar uthandi department of agricultural microbiology, tamil nadu agricultural university, coimbatore 641 003, tamil nadu, india corresponding author a b s t r a c t coconut and coco medium. Text is available under the creative commons attributionsharealike license. The fungus has wide range of plant hosts and known to cause yield losses up to 80% especially on cash and food crop farms. Archives of phytopathology and plant protection,112. A description is provided for botryodiplodia theobromae. Postharvest treatment of fruit with hot water or fungicides can reduce the development of this disease. Botryodiplodia theobromae may not be the main causal organism of this disease.
Tubers were incubated for seven days only with botryodiplodia theobromae because of rapid deterioration in a crd experiment of two treatments the two markets, lagos and abeokuta and seven replicates. Proceedings of an international workshop held at chiang mai, thailand, 1821 may 1997. Mango dieback and gummosis in sindh, pakistan caused by. Botryodiplodia theobromae is a virulent plant pathogen commonly found in the tropics and subtropics. But, it is an opportunistic pathogen and becomes more virulent in combination with others fungi, i. Identification and pathogenicity of lasiodiplodia theobromae. Citrus is one of the most important fruit crops in florida and in the world. Metabolites produced by botryodiplodia theobromae synonym lasiodiplodia theobromae possessing plant growth regulating activities were shown to be the 7epimer of jasmonic acid and its derivatives. In this study, 53 actinobacterial isolates were obtained from mango rhizosphere soil in the uae, of which 35 66% were classified as streptomycetes sa and 18 34% as nonstreptomycetes nsa. Lasiodiplodia theobromae in the root rot disease complex of rice. Introduction botryodiplodia theobromae pathogen fungi grow on diverse. Morphological, pathogenic and molecular characterization of lasiodiplodia theobromae. Metabolites produced by botryodiplodia theobromae synonym lasiodiplodia theobromae. Morphological, pathological and molecular variability in.
First report of lasiodiplodia theobromae causing needle. A partial list of the recorded hosts includes citrus. Infection occurred following inoculations made throughout the year, but was most common when the fungus was introduced during warm weather. Peran mikoriza arbuskular, kitosan, dan trichoderma harzianum. Old or inactive cankers appear sunken and are surrounded by callus tissues. Since the introduction of cocoa theobroma cacao in cameroon in 1886, the main disease has been phytophthora pod rot. Pdf morphological and molecular data reveal cryptic species. The conidia of botryodiplodia theobromae are initially hyaline and nonseptate and may be released from pycnidia in this condition.
Mango mangifera indica is a major tropical fruit species cultivated in brazil. We report a case of lasiodiplodia theobromae pneumonia in a patient who died 14 days after cadavericliver transplantation. Morphological and molecular characterization of lasiodiplodia theobromae from various banana cultivars causingcrown rot disease in fruits. Botryodiplodia theobromae not known to produce onychomycosis was repeatedly recovered from a healthy woman with evident lesions in a toe nail. Penyakit diplodia botryodiplodia theobromae pada tanaman jeruk menyebar cukup luas di sentra jeruk indonesia. Percentage rot of banana fruits was found to be maximum at 25 c and 30. Plurivorous, on approximately 500 host plants 30, 181. Mycclial fragments were observed in the scales and the fungus was isolated in cycloheximide free culture media. Dieback due to lasiodiplodia theobromae, a new constraint. Dieback due to lasiodiplodia theobromae, a new constraint to. Thirteen isolates of botryodiplodia theobromae collected from pear varieties grown in various regions of punjab were studied for morphological, pathological. Pdf morphological, pathological and molecular variability in. Pdf morphological and molecular data reveal cryptic.
Five cellulases c1, c2, c3, c4 and c5 were found, and their molecular. His condition was complicated by enterococcus faecium peritonitis. Sycamore canker caused by botryodiplodia theobromae. It causes rotting and dieback in most species it infects. This page was last edited on 14 august 2019, at 00. The hydrolysis of onitrophenyl betadglucopyranoside by the highmolecularweight betaglucosidase betadglucoside glucohydrolase, ec 3.
It also infects biancaea sappan, a species of flowering tree also known as sappanwood. Extensive tests of seed collected from pods on infected. The effect of glycerol on the activity of betaglucosidase. Botryodiplodia theobromae, is the major causal organism of soursop fruit. Lasiodiplodia theobromae was identified based on morphological characters as described by punithalingam 1976. Germination of conidia of botryodiplodia theobromae in. As disease agent, pathogen is encountered in its anamorph state, named as lasiodiplodia. Botryodiplodia theobromae was isolated from the bark samples. Control of botryodiplodia theobromae rot of white yam. Morphological and molecular data reveal cryptic speciation. This disease has been reported in hawaii and india only, but unidentified botryodiplodia spp. Conclusion depending on the technique adopted, each of t. Molecular characterization of cocoa, mango, banana and yam. This finding is important in the study of management options for future prevention and.
Pathogenic association and management of botryodiplodia theobromae in guava orchards at sheikhupura district, pakistan. Evaluation of jasmonic acid production by lasiodiplodia. Botryodiplodia theobromae grew and sporulated best at 28c on media amended with ripe mangoextract and dextrose or sucrose. References this ascomycotarelated article is a stub. Botryodiplodia theobromae and diplodia natalensis is an economically important postharvest fruit decay that occurs on all types of citrus grown in florida and other hot, humid tropical and subtropical regions in the world. This disease can lead to large losses as it can attack plants from the nursery until the time of production. The objective of this study was to identify species of lasiodiplodia associated with dieback and stemend rot of. Lasiodiplodia theobromae, test their virulence and evaluate different fungicides to find out effective ones for. Pdf species of lasiodiplodia associated with mango in brazil. On rare occasions it has been found to cause fungal keratitis. Dieback caused by the fungus lasiodiplodia theobromae is an important disease on mango plantations in the united arab emirates uae.
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